Chow Chi Fai graduated from the Chinese Department, Hong Kong Baptist University in 1979. He joined the HKRep as part-time actor at the same year and became a full time actor in 1981.

Chow played in over 200 productions, including Seventy-two Tenants, Confucius, Jesus Christ and John Lennon of the Beatles, Antigone, The Government Inspector, Absurd Person Singular, I Have A Date With Spring, Small Ripples In A Dead Pool, Crucible, Tales Of The Walled City, Desire Under The Elm, When I Was Deserted, Uncle Vanya, Secret Of Resurrection, A Dream Like A Dream, Forever & Ever, Rape Virus, The Eccentricities Of A Nightingale, Departure 00:00, A Small Family Business, Rotate 270° ,Vassa Zheleznova, Zheng He and The Emperor, Proof, , (Rep 2 Series) Do You Still Remember, Angel in the Box and Peter Pan.
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Chow was nominated the Best Supporting Actor (Tragedy/Drama, and Comedy/Farce respectively) at the 3rd Hong Kong Federation of Drama Societies’ Hong Kong Drama Awards for his work of Master Kiang in The Legend of the Mad Phoenix, and Andrew Jorgenson in Other People's Money in 1994. He was nominated Best Actor (Tragedy/Drama) for his work of Qu Yuan in The Story of Qu Yuan and for Ching-wa in Before the Rain Stops in the 7th Hong Kong Drama Awards in 1998. He won the award for The Story of Qu Yuan and for The Legend of the Mad Phoenix. In 2004 he was awarded Nomination award (Best Actor) for his work of Uncle Fu in Sweet and Sour Hong Kong at the 15th Shanghai Magnolia Stage Award and he was nominated Best Supporting Actor(Comedy/Farce) for his work of Lady Bracknell in The Importance of Being Earnest in 2005. At 15 th HK Drama Awards In 2006,he was nominated Best Supporting Actor(Tragedy / Drama) for his work of Robert in Proof.

Chow directed the school touring productions Checkpoint and Sunset Sparks. He also played in over 20 movies and appeared frequently in the Radio Television Hong Kong Education Television programs. An experienced voice-dubbing artist, Chow's credits include The Archdeacon in The Hunchback of Notre Dame, Buzz Lightyear in the Buzz Lightyear movie and cartoon series, the designated HK Cantonese dubbing artist of Captain Hook in Peter Pan. Other dubbing credits include Pocahontas, Mulan and Disney on Ice.